Here is what you can do to maximize your Depth of Field and reduce constant focusing:
1. Initial focus should be done at the highest magnification
2. This is called “Parfocality” and this diagram shows how it works:
Explanation: if you focus on object #1 at low magnification (2x) your object will out of focus at 12x high magnification. If you start at high magnification and focus on object #2, you will be in focus throughout the range of magnification (#3).
The other action you can initiate is to reduce the amount of light into the microscope through aperture (iris) adjustment. Reducing the illumination can enhance the DOF. To do this, most microscopes have auto iris and some have an additional manual iris.
Haag-Streit Surgical Microscopes have the best depth of focus allowing more anatomy in focus. For additional information, click on these links:
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